root (hd0,0) - Configures the root partition for GRUB, such as (hd0,0) first hard disk, first Partition and mounts the partition.
kernel /vmlinuz-i686-up-4GB root=/dev/hda9 - Specifies the kernel location which is inside the /boot folder. This location is related to the root(hd0,0) statement.The root partition is specified according to the Linux naming convention (/dev/hda9/)
A imagem do Kernel será montada no sistema de arquivos raiz (/ dev / hda9)